Comparative Performance and Microbial Community of Single-phase and Two-phase Anaerobic Systems Co-Digesting Cassava Pulpand Pig Manure

Abstract

In this study, we illustrated the performance and microbial community of singleand two-phase systems anaerobically co-digesting cassava pulp and pig manure. The results showed that the volatile solid reduction and biogas productivity of two-phase CSTR were 66 ± 4% and 2000 ± 210 ml l d, while those of singlephase CSTR were 59 ± 1% and 1670 ± 60 ml l d, respectively. Codigestion in two-phase CSTR gave higher 12% solid degradation and 25% methane production than single-phase CSTR. Phylogenetic analysis of 16S rDNA clone library revealed that the Bacteroidetes were the most abundant group, followed by the Clostridia in singlephase CSTR. In hydrolysis/acidification reactor of two-phase system, the bacteria within the phylum Firmicutes, especially Clostridium, Eubacteriaceae and Lactobacillus were the dominant phylogenetic groups. Among the Archaea, Methanosaeta sp. was the exclusive predominant in both digesters while the relative abundance of Methanosaeta sp. and Methanospirillum hungatei differed between the two systems. Keywords—Anaerobic co-digestion, Cassava pulp, Microbial diversity, Pig manure.

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